sybr gold,

🗣 SL提问: sybr gold

✍️ Ai批改: SYBR Gold is a fluorescent nucleic acid stain commonly used in molecular biology for the detection and quantification of DNA、Here are some key points about SYBR Gold:

1、Function:
It binds to double-stranded DNA with high sensitivity.
Used for staining agarose gels after electrophoresis.

2、Characteristics:
Fluoresces brightly when bound to DNA
Has low background fluorescence when unbound
Can be used for both gel staining and in-situ hybridization

3、Advantages:
High sensitivity (can detect as little as 0.5 ng of DNA)
Broad excitation and emission spectra, making it compatible with many imaging systems
Non-toxic alternative to ethidium bromide

4、Applications:
Gel staining for visualization of DNA fragments
Quantitative analysis of DNA
Live cell imaging (with appropriate filters)

5、Excitation/emission wavelengths:
Excites at around 494 nm
Emits at around 523 nm

6、Usage:
Post-gel staining
In-gel staining
Microarray analysis
Flow cytometry

7、Safety:
Less mutagenic than ethidium bromide
Can be used with UV or blue light transilluminators

8、Mechanism:
Intercalates between base pairs of DNA
Increases fluorescence upon binding to DNA

9、Preparation:
Typically diluted from a stock solution before use
Can be used with various imaging systems

10、Considerations:
More sensitive than ethidium bromide
Can be used with UV or blue light sources

11、Limitations:
Not specific to DNA, also binds to RNA and single-stranded DNA
May interfere with some downstream applications due to its strong binding affinity

12、Storage:
Usually stored in the dark at room temperature

13、Handling:
Should be handled carefully, as it can still be toxic if ingested or absorbed through skin

14、Compatibility:
Works well with most common imaging systems

15、Alternative names:
Sometimes referred to as SYBR Safe or SYBR Green I in some contexts

16、Related products:
SYBR Green I: similar but optimized for real-time PCR

17、Precautions:
Although less toxic than ethidium bromide, still requires careful handling

18、Availability:
Commercially available from various suppliers like Invitrogen/Life Technologies

19、Cost-effective compared to other stains

20、Comparison:
Often preferred over ethidium bromide due to lower toxicity

21、Usage:
Dilute according to manufacturer's instructions
Avoid prolonged exposure to light to prevent photobleaching

22、Imaging:
Requires appropriate filter sets for visualization

23、Alternatives:
Other dyes like SYBR Green I for qPCR

24、Benefits:
Safer than ethidium bromide
Long-lasting signal

25、Specificity:
Nonspecific binding to all nucleic acids

26、Protocol:
Follow safety guidelines during handling

27、Detection:
Visualized under UV or blue light

28、Sensitivity:
Detects down to nanogram levels of DNA

29、Versatility:
Suitable for various types of DNA samples

23、Documentation:
Check protocols for optimal results

24、Research:
Widely used in research labs for routine DNA analysis